Ruypeptide Biotechnology Co., Ltd. is one of the most reliable manufacturers and suppliers of high purity klow in China. If you're going to buy high quality high purity klow made in China, welcome to get quotation from our factory. Also, discount information is available.
Core Composition & Physicochemical Specifications
| Item | Details |
|---|---|
| Blend Components | GHK - Cu (50 mg)+BPC - 157 (10 mg)+TB - 500 (10 mg)+KPV (10 mg); total 80 mg |
| Individual Peptide Purity | ≥98% (HPLC); blend overall purity ≥99% |
| Formulation | White lyophilized powder; reconstituted with bacteriostatic water/PBS |
| Solubility | Soluble in water (≥20 mg/mL) after reconstitution; pH 6.0 - 7.4 optimal |
| Stability | Stable at - 20°C for 2 - 3 years (sealed, light - protected); reconstituted solution stable at 2 - 8°C for 1 month |
| Key Quality Metrics | Endotoxin ≤0.1 EU/mg; moisture ≤0.5%; heavy metals ≤10 ppm |
Mechanism of Action (Synergistic Effects)
The blend's efficacy stems from the complementary actions of its four components:
GHK - Cu: Stimulates collagen synthesis, enhances antioxidant defense, and promotes skin/tissue remodeling; acts as a copper - dependent signaling molecule to regulate gene expression related to tissue repair.
BPC - 157: Accelerates angiogenesis, boosts fibroblast migration, and strengthens tissue integrity; protects against gastrointestinal and musculoskeletal damage by modulating growth factor signaling.
TB - 500: Facilitates cell migration and actin polymerization, reduces fibrosis, and accelerates the healing of tendons, muscles, and other soft tissues.
KPV: Blocks CXCR2, inhibits NF - κB/MAPK pathways to reduce inflammation, and supports wound closure by promoting keratinocyte migration without excessive scarring.
Synergy: GHK - Cu/KPV addresses inflammation and collagen production; BPC - 157/TB - 500 enhances repair and angiogenesis, creating a comprehensive regenerative environment.
Biological Functions & Application Fields
Core Physiological Effects
Skin Regeneration: Improves skin elasticity, reduces wrinkles, and accelerates wound healing by promoting collagen/elastin synthesis and reducing inflammation.
Soft Tissue Repair: Enhances healing of muscles, tendons, and ligaments; reduces post - injury fibrosis and scarring.
Anti - Inflammatory: Inhibits pro - inflammatory cytokine release (TNF - α, IL - 6), alleviating localized and systemic inflammation.
Gastrointestinal Protection: BPC - 157 component supports gut barrier function and heals mucosal lesions.
Application Fields
| Field | Usage Scenarios |
|---|---|
| Preclinical Research | Studying tissue regeneration, wound healing, and inflammatory pathways in rodent models. |
| Skin Research | Evaluating anti - aging, wound - care, and post - inflammatory hyperpigmentation treatments. |
| Musculoskeletal Research | Investigating soft - tissue injury repair and fibrosis reduction strategies. |
| Topical Formulation Development | Developing transdermal blends for localized tissue repair studies. |
Production & Quality Control
Manufacturing Processes
Individual Peptide Synthesis: Each peptide (GHK - Cu, BPC - 157, TB - 500, KPV) is synthesized via SPPS (Fmoc chemistry) and purified by RP - HPLC to ≥98% purity.
Blending & Lyophilization: Components are mixed in precise ratios, lyophilized to form a stable powder, and vacuum - sealed with desiccant.
Quality Validation: Each batch undergoes identity (mass spectrometry), purity (HPLC), endotoxin (LAL), and moisture (Karl Fischer) testing before release.
Quality Testing Standards
| Test Item | Specification | Detection Method |
|---|---|---|
| Purity (Individual Peptides) | ≥98% | RP - HPLC (C18 column, gradient elution) |
| Identity | Matches reference standards | Mass spectrometry, amino acid analysis |
| Endotoxin | ≤0.1 EU/mg | Limulus Amebocyte Lysate (LAL) test |
| Moisture | ≤0.5% | Karl Fischer titration |
| Heavy Metals | ≤10 ppm | ICP - MS |
Administration, Safety & Regulatory Status
Administration Routes & Dosage
Preclinical Injection: Subcutaneous (SC) injection (0.2 mL reconstituted solution, 20 - 50 μg/kg, 5 times/week for 3 weeks); intraperitoneal (IP) injection (1 - 5 mg/kg/day).
Topical Use: 0.5% - 1% blend in cream/serum, applied twice daily for localized studies.
Cell Culture: 1 - 10 μM for in vitro studies on cell migration and inflammation.
Safety Profile
Low Toxicity: No systemic toxicity reported in rodent studies; individual components have LD₅₀ > 100 mg/kg.
Minimal Side Effects: Transient local irritation at injection sites; no off - target organ damage observed.
Contraindications: Hypersensitivity to any peptide component; avoid use in pregnant/lactating animals (limited data).
Research Use Only: Not approved for human clinical use; for laboratory research applications only.
Handling Guidelines
Storage: Store lyophilized powder at - 20°C; reconstituted solution at 2 - 8°C for up to 1 month.
Reconstitution: Use bacteriostatic water; avoid repeated freeze - thaw cycles to prevent peptide degradation.
Transport: Cold chain transportation (2 - 8°C) to maintain stability.
Key Advantages & Limitations
Advantages
Synergistic Efficacy: Combines four peptides to address regeneration, inflammation, and repair simultaneously, outperforming single - peptide treatments.
Multi - Tissue Application: Suitable for skin, musculoskeletal, and gastrointestinal research, offering broad utility.
Cost - Effective: Pre - blended format reduces synthesis and testing costs for research labs.
Well - Characterized: Each component has extensive preclinical data, supporting mechanism - of - action studies.
Limitations
pH Compatibility Issues: GHK - Cu (optimal pH 5.5 - 6.5) and TB - 500/KPV (optimal pH 7.0 - 7.4) may have stability conflicts in a single formulation.
Short Half - Life: Rapid in vivo clearance requires frequent dosing or sustained - release formulations.
Preclinical Only: Limited human data; clinical translation requires further safety and efficacy studies.
1. Molecular & Physical Properties
Amino Acid Sequence: Ala-His-Lys-Cu, formed by chelating the tripeptide alanyl-histidyl-lysine with copper ions.
Purity: ≥99% (HPLC verified), ensuring high bioactivity and minimal impurities.
Physical Appearance: Deep blue lyophilized powder, highly water-soluble for easy formulation into solutions, creams, or injectables.
Stability: Optimal storage at -20℃; stable for 24 months when sealed from light and moisture; avoid high temperatures to prevent copper ion dissociation.
2. Core Biological Mechanisms
Collagen & Elastin Synthesis: It upregulates the expression of TGF-β and matrix metalloproteinase inhibitors (TIMPs), stimulating fibroblasts to produce type I/III collagen and elastin. This enhances skin elasticity, reduces fine lines, and improves skin texture.
Antioxidant & Anti-inflammatory Effects: The copper ion moiety scavenges free radicals (e.g., superoxide anion, hydroxyl radicals) to mitigate oxidative stress. It also inhibits NF-κB pathways, reducing the release of pro-inflammatory cytokines (TNF-α, IL-6) and alleviating tissue inflammation.
Hair Follicle Activation: It extends the anagen (growth) phase of hair follicles, increases dermal papilla cell proliferation, and improves microcirculation around hair follicles, which aids in reversing hair miniaturization associated with androgenetic alopecia.
Wound Healing Acceleration: By promoting angiogenesis and fibroblast migration, it speeds up granulation tissue formation and epithelialization, shortening recovery time for acute and chronic wounds (e.g., burns, diabetic ulcers).
3. Application Scenarios
Dermatological Cosmetics: Added to anti-aging serums, creams, and masks to improve skin firmness, fade wrinkles, and repair photodamaged skin.
Trichological Research: Used in topical formulations or scalp injections for androgenetic alopecia treatment and hair regrowth studies.
Regenerative Medicine: Applied in wound care dressings and tissue engineering scaffolds to enhance skin and soft tissue repair.
Laboratory Research: Serves as a tool compound for studying copper peptide biology, oxidative stress, and tissue regeneration mechanisms.
4. Safety & Advantages
Non-immunogenic, with no reported allergic reactions or systemic side effects in topical use.
Biodegradable, breaking down into natural amino acids and copper ions that are metabolized by the body.
Compatible with most cosmetic and pharmaceutical excipients (e.g., hyaluronic acid, glycerin), suitable for multi-component formulations.
Hot Tags: high purity klow, China high purity klow manufacturers, suppliers
